FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46460506
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA132750.015.91251845
TATATAC71250.013.5824637
AGCTTAG172250.013.36054845
GGGAGGT242500.013.0991463
ATAGGGG178400.013.09898438
GGCATAG174750.013.0769835
GGAGGTC240450.012.4733634
AGGGATC131250.012.2554192
ATATATA71000.012.0445266
GTTGTCG30800.011.98105926
GAGCTTA193900.011.9725444
AGGTCAC251650.011.9474166
GAGGTCA257950.011.8552675
GTCACTC244500.011.7557458
CACCGAA329850.011.64192745
CATAGGG197300.011.57099937
CATGCAT100300.011.489669
GTTCTAT188850.011.47967151
GCACCGA335200.011.38838944
ATATAGC38950.011.3821226
Produced by FastQC (version 0.11.5)