FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58772022
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG590660.10050020058864062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG34200.026.0516266
TCACGCT272200.021.3089128
CGGTATA56650.020.17624320
AACGCTA280000.020.16132723
ACGCTAC280100.020.15412924
TCGACGA46650.019.8706177
CGCTACC294250.019.13907225
TTCACGC311650.019.037457
CACGCTC316450.018.3441569
CGACGAG50000.017.8643428
ACGCTCA346800.017.34379210
AAAACGC330500.017.10793321
AGACCGC106800.016.9166854
GAAAACG343600.016.51461420
AAACGCT351350.016.15033722
GACCGCG111650.016.1415315
ACCGCGT112450.015.966486
GTTTTCG354550.015.87766645
GTCCGTT52350.015.6903981
CGTGGTT361000.015.50050441
Produced by FastQC (version 0.11.5)