FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58772022
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG724970.12335291101606134No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA610640.10389977734643874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG212050.017.06149545
GTTGTCG31400.015.11903426
GGCATAG243350.015.04334535
ATAGGGG243550.014.97553538
GTCACTC344900.014.5603758
GAGCTTA257250.014.21239844
GGGAGGT388400.014.0132883
GGAGGTC378500.013.7138094
TGGCATA269000.013.66691934
GGTCACT372500.013.554027
GAGGTCA385550.013.4688685
AGGTCAC393700.013.2298966
ACTCTCC379800.013.20458711
CATAGGG294400.012.51882537
GCATAGG307750.012.01963336
TCACTCT431250.011.7544879
CACCGAA283700.011.68981245
TCACCCG103150.011.4299793
ATATAGC50400.011.071136
TGGGAGG365250.010.9283672
Produced by FastQC (version 0.11.5)