FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54857685
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1283780.23402008305673125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT264600.025.57779145
ACCGCGT310350.022.4096056
AGACCGC314250.022.231594
GACCGCG314600.022.1353345
CCGCGTT322550.021.568757
CGCGTTC323850.021.4404878
AGAACGA319750.021.20135544
CAGACCG331650.021.1598823
TTCGACG20350.020.4547546
TCAGACC371000.019.485622
AATACGG356200.019.42967232
TACGGAG355450.019.27444534
ATACGGA359400.019.26919633
GCGTTCT377750.018.470559
AAGAACG386700.018.09511243
CAATACG385250.018.0696931
CTCAGAC457450.017.3172591
ACGGAGA397300.017.31210735
CCCAATA420200.016.78102129
CGGAGAG423100.016.1181836
Produced by FastQC (version 0.11.5)