FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54857685
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG696090.1268901522184175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG223850.015.75303445
ATAGGGG256700.013.90489138
GGCATAG253400.013.81856835
GTCACTC346500.013.5837248
GAGCTTA266800.013.43782744
GGGAGGT390900.013.1298533
GGAGGTC375350.012.937644
GGTCACT363600.012.8652917
ACTCTCC372650.012.61508111
AGGTCAC382550.012.5684786
TGGCATA283100.012.5456434
CATAGGG284000.012.48010337
GAGGTCA402600.012.2513195
GCATAGG305000.011.70207136
TCACTCT423000.011.2714269
ACCCGGC95100.011.07685855
CACTCTC428600.010.95114110
GTTGTCG28400.010.91604826
TAGGGGA432500.010.87189839
TGGGAGG362000.010.6288862
Produced by FastQC (version 0.11.5)