FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48156848
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG610360.1267441756154805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG209350.014.80707845
GGCATAG211400.014.6191935
GGAGGTC297350.014.4752824
ATAGGGG216300.014.4329838
GGGAGGT311050.014.2770963
GTCACTC304450.013.6469918
GGTCACT307850.013.436427
GAGCTTA242400.012.96404844
TGGCATA244150.012.76765134
GTTGTCG28500.012.55342126
ACTCTCC330800.012.46556311
CATAGGG253900.012.27842137
GCATAGG267950.011.60101236
AGGTCAC388300.011.1966126
GAGGTCA401950.010.8941835
TGGGAGG310800.010.8577322
CACTCTC386500.010.69822110
TGTCGTT35250.010.66004628
TAGGGGA390850.010.57115739
TCACTCT405250.010.5079819
Produced by FastQC (version 0.11.5)