FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46373266
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1180040.25446557937066583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT292100.023.0010491
CCGGCTT333050.021.6314262
TCACGCT329850.021.094578
TTCGACG26400.020.5362136
TTCACGC343500.020.3807477
ACGCTCA365300.018.99821310
CGGCTTC391850.018.8677863
AACGCTA371150.018.3311723
ACGCTAC370950.018.31078324
CGCTACC378650.017.92735725
CACGCTC387850.017.8936359
AAAACGC392350.017.44423121
GAAAACG401500.016.88930520
AAACGCT408200.016.77196522
TCGTGGT421150.015.78558640
CGTGGTT420650.015.77293941
CGCTCAG445050.015.75058911
GTTTTCG430600.015.49222745
AGACCGC50350.015.4611544
CGGTATA44750.015.38446820
Produced by FastQC (version 0.11.5)