FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46373266
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA621380.13399530669243784No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG551760.11898234642347598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT301850.014.3425563
GGAGGTC293450.014.1087024
AGCTTAG216700.013.57050945
GTCACTC301800.013.1062138
ATAGGGG231500.013.08329338
GAGGTCA322000.012.9694545
AGGTCAC323800.012.7167936
GGCATAG239300.012.57219935
GGTCACT320400.012.4719167
ACTCTCC326850.012.08767711
GTTGTCG22650.011.81988426
CACCGAA288650.011.75463745
GAGCTTA255650.011.61812844
TCACTCT346900.011.5060669
TGGCATA263200.011.43057434
GCACCGA273550.011.0964344
TGGGAGG294800.010.9856942
CACTCTC360350.010.86410210
GCGTCGT28700.010.50484711
CATAGGG287400.010.48379937
Produced by FastQC (version 0.11.5)