FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54780813
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT776620.1417686152266488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT183850.021.930686
GACCGCG183950.021.8819665
AGACCGC189950.021.285364
CCGCGTT192500.020.8984727
CGCGTTC195300.020.5988528
CAGACCG198250.020.4850673
GAACGAT211550.018.94449245
TTCGACG25300.017.5195716
TCAGACC246150.016.9192072
GCGTTCT246600.016.4687989
AGAACGA254400.015.91266744
TCGGCGC73200.015.83172445
ATACGGA254800.015.70016233
AATACGG254150.015.68719932
TACGGAG259450.015.384088534
CCCGTAT35500.015.0288221
CTCAGAC316800.014.7021281
TTCGGCG83150.014.34308244
CAATACG281000.014.2443131
GTCCGTT43850.014.220511
Produced by FastQC (version 0.11.5)