FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54780813
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG163700.015.14580345
ATAGGGG182150.013.94738438
GGCATAG178350.013.806313535
GAGCTTA193500.012.99096844
CATAGGG189850.012.93321137
GTCACTC252000.012.35218
GCATAGG202350.012.19927636
ACCCGGC86750.012.1434185
GGTCACT260450.011.9086077
GGGAGGT300300.011.7368083
TGGCATA212300.011.7026734
ACTCTCC283950.011.31961811
GGAGGTC290700.011.1893784
AGGTCAC290200.011.0899216
GAGGTCA311550.010.5187735
ACGGACA103400.010.42954418
TAGGGGA310550.010.12796939
ATAGCTC98300.09.892739
CGGACAC107400.09.82987113
TCACTCT336000.09.518269
Produced by FastQC (version 0.11.5)