FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47510226
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT782650.16473295664811194No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG734040.1545014751140102No Hit
GCCAGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG574960.12101815722787763No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT481630.10137396525960539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA293250.025.22696323
ACGCTAC293750.025.19184124
AGACCGC157250.024.6595824
CGCTACC302200.024.42788925
TCACGCT314150.024.135758
ACCGCGT167550.023.1436566
AAAACGC320550.023.13461721
CAGACCG169900.022.916213
GACCGCG170350.022.7236375
GAAAACG326750.022.68875920
TTCACGC337950.022.509217
AAACGCT333150.022.25964722
CACGCTC352950.021.4888659
ACGCTCA358250.021.4472110
CCGCGTT181500.021.4020237
CGCGTTC182300.021.3081048
GAACGAT182900.021.0971845
GTTTTCG328150.020.44604145
CGTGGTT373250.019.89223141
TCGGCGC108200.019.8170845
Produced by FastQC (version 0.11.5)