FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47510226
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1122970.2363638514369517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG302650.018.16806245
GGGAGGT404350.017.8081873
GTCACTC397900.017.343458
GGCATAG320300.017.2813835
ATAGGGG326650.017.12126738
GGAGGTC412950.017.0549454
GGTCACT410000.016.8480347
ACTCTCC415000.016.8186711
AGGTCAC434400.016.2590566
GAGCTTA340950.016.21409644
TGGCATA344300.016.12129634
TGGGAGG360200.015.9241942
GAGGTCA446450.015.8860495
CATAGGG370850.015.05031337
GCATAGG388800.014.36705336
TCACTCT487200.014.3077449
CTGGGAG451900.014.0160181
CACTCTC495850.013.99008210
GTTGTCG26550.013.47233226
GCTCTGT525650.013.33563622
Produced by FastQC (version 0.11.5)