FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53268419
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG886440.16641004494614342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG247350.017.22584245
ATAGGGG272050.015.78311338
GGCATAG271850.015.57817535
GGGAGGT395200.015.3883853
GGAGGTC387350.015.1193984
GAGCTTA291400.014.7145744
GTTGTCG35450.014.15156426
TGGCATA301450.014.13801934
ACTCTCC420950.013.82643511
GGTCACT419150.013.7300127
GTCACTC429300.013.5309238
CATAGGG317900.013.41409337
AACTCTA115650.012.78678845
GCATAGG337700.012.60728736
TGGGAGG379450.011.9477252
CACCCGG118250.011.9107851
TAGGGGA484600.011.83826839
CACTCTC490800.011.82642310
ATATAGC48650.011.4686416
TGTCGTT43950.011.41462828
Produced by FastQC (version 0.11.5)