FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40634516
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG14450.020.395686
GTCCGTT31550.019.9158971
ATGCGCG24450.017.75978744
TGCGCGT24250.017.62792445
CTCGCGT8500.017.4871921
CTCGGTT90000.017.1162511
AGACGCT164700.016.16035741
CCCCGCA156400.016.02531835
GTTCCTA83050.015.90210315
ATGTCGC86900.015.89669335
CTCCGTT64950.015.7771041
AAGCGGC157050.015.69058528
CCGCAGA175300.015.59320737
GTCCGAT22150.015.4548551
GTCGGTT31450.015.2529561
CTCCGAT46250.015.0467191
TTCGACG15900.014.9983486
CGGTTTT92300.014.7464813
GCAGACG180400.014.70405439
AACGCTA54950.014.65869923
Produced by FastQC (version 0.11.5)