FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48703766
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCA627000.12873747791905865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG35900.016.2296926
ACAGACT229200.015.9323052
AGACTCA255200.014.6894254
CACAGAC289800.013.7012211
CCGACAA242700.013.58192540
AGCTTAG94550.013.49425845
TGTCGTT45300.013.30622128
CAGACTC282200.013.2587423
GCCGACA253450.013.09162239
GGCATAG97300.013.06139435
ACCCGGC94800.012.7465075
ACTCAGA302550.012.5931866
GACTCAG304050.012.5666045
ATAGCTC93300.012.20007739
ATAGGGG108100.012.00676338
TCACCCG105700.012.005253
GCTAAGT50050.011.87279914
ATATAGC56650.011.6397476
GTCGTTC51800.011.54956229
AACCCAC296500.011.41727616
Produced by FastQC (version 0.11.5)