FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45632760
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT962320.21088358451253003No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG517650.11343824042201262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA140550.024.62152323
AGACCGC226900.024.4367964
ACCGCGT227100.024.386236
GACCGCG229800.024.099765
ACGCTAC143350.024.07955624
CAGACCG236400.023.5598323
GAACGAT241400.022.95732745
CCGCGTT242600.022.828247
CGCTACC153000.022.62279125
CGCGTTC246500.022.467048
AAAACGC160200.021.70902321
TCAGACC273900.020.925622
TTCGACG21750.020.477636
GAAAACG174250.019.91862120
AATACGG276050.019.91613832
AAACGCT177850.019.64580722
ATACGGA282200.019.5215733
AGAACGA286200.019.50795644
GCGTTCT286000.019.5058829
TACGGAG278650.019.48015434
Produced by FastQC (version 0.11.5)