FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45632760
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG523700.11476404232397953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG176100.015.51304345
GGCATAG190650.014.29074535
GTTGTCG28750.013.851459526
GAGCTTA207350.013.29535744
ATAGGGG211550.013.23055938
TGGCATA214450.012.84079334
GGGAGGT333300.012.486633
CATAGGG223450.012.41518937
ACTCTCC302250.012.078691511
GTCACTC309650.011.986028
GGTCACT311100.011.9376487
GCATAGG235850.011.79138636
GGAGGTC325800.011.7811214
AGGTCAC342400.011.27956
TGTCGTT38500.010.96017528
ACCCGGC85550.010.94730955
ATATAGC41450.010.8884656
TAGCGTA10300.010.72969537
GAGGTCA363450.010.7274475
ATAGCTC74900.010.6899639
Produced by FastQC (version 0.11.5)