FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73005419
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1194810.16366045375343985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT234600.024.57739645
ACCGCGT274900.020.9507266
AGACCGC276600.020.7893564
ACGTTCG44250.020.2858626
GACCGCG284800.020.2618665
AGAACGA286850.020.18700644
CCGCGTT287050.020.0317547
CGCGTTC295550.019.4787718
CAGACCG300550.019.3507143
AATACGG311850.018.5113832
ATACGGA317000.018.21106733
TACGGAG325950.017.76644734
ACGCTAC93450.017.57367124
AACGCTA92450.017.5203623
TTCGGCG113000.017.47338144
TCAGACC353050.016.728232
GCGTTCT362000.015.9901849
GTCCGTT54300.015.9737011
CAATACG363250.015.95980131
AAGAACG371100.015.63437243
Produced by FastQC (version 0.11.5)