FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73005419
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG183850.014.53992545
ACCCGGC163500.013.6946645
GGCATAG194900.013.63672635
ACGGACA176050.012.65268118
TCACCCG189000.012.6200623
CGGACAC186950.012.285274513
ATAGCTC177000.012.21882239
CCGGACA189800.012.14843512
TAGCATA121600.012.0635979
CATAGGG222550.012.02329137
CGGACAG185150.011.8848219
GAGCTTA235750.011.692800544
ACACGGA190450.011.59922916
ATAGGGG233150.011.54444938
GCATAGG233550.011.47640136
TGGCATA236400.011.40363134
GTTGTCG34950.011.13532626
CACCCGG214850.010.9342614
GACACGG205650.010.78536615
TAGCTCT200600.010.74808540
Produced by FastQC (version 0.11.5)