FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50471784
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG720930.14283822422444986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG33950.024.6536056
TCGACGA38100.022.6768917
CGGTATA55800.022.45936220
GACCGCG70600.021.7348655
ACCGCGT73400.020.8444356
AGACCGC76400.020.1438584
CGACGAG41750.019.670428
AACGCTA194400.019.5599123
ACGCTAC196850.019.32789624
CGCTACC203650.018.58309225
AAAACGC213250.017.98919521
CAGACCG88800.017.8884033
TCACGCT231200.017.3224548
GAAAACG223250.017.1229420
GCCGGCT227600.016.6044961
AAACGCT232800.016.507522
ACGCTCA249150.016.20085510
CCGCGTT95050.016.0965967
CCGGCTT261750.015.988442
TTCACGC255150.015.8287337
Produced by FastQC (version 0.11.5)