FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47368879
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1048370.22132041587895718No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG897780.1895295010042353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC208200.025.4719814
CAGACCG215100.024.686273
TTCGACG13600.020.8399776
GAACGAT259350.020.14790545
GCGTTCT264150.020.113739
ACCGCGT266900.019.8981656
GACCGCG268850.019.7496835
CTCAGAC318550.019.664121
CCGCGTT279450.019.0286947
GCCGGCT249500.018.9155311
TACGGAG275550.018.74834434
ATACGGA280050.018.6324733
CGCGTTC285550.018.61438
AATACGG281050.018.51010732
AGAACGA287200.018.22547744
TCACGCT294350.018.1571458
ACGGAGA296200.017.58539235
CAATACG305600.017.17780931
TCAGACC323350.016.9924762
CTACACG121700.016.94906445
Produced by FastQC (version 0.11.5)