FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50662260
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT769870.15196124294494562No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG592540.1169588565531818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC163650.024.3005714
ACCGCGT166250.023.8727056
GACCGCG166800.023.7877165
CCGCGTT173950.022.8159667
CAGACCG177800.022.6196543
CGCGTTC176300.022.523848
GAACGAT180700.021.56822645
AACGCTA158900.020.29553223
ACGCTAC160550.020.17141724
CGCTACC166950.019.35792525
ATACGGA202800.019.09309633
TCAGACC217000.018.9797842
TACGGAG204150.018.92264234
AATACGG205300.018.8837632
AGAACGA209050.018.72845844
AAAACGC182550.017.78829821
GCGTTCT228650.017.4751229
TCACGCT198000.017.2275878
CAATACG227700.017.21378731
CTCAGAC281050.016.915271
Produced by FastQC (version 0.11.5)