FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51343435
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG709120.13811308105895137No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT560750.10921552093271515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG34700.027.5572836
GTTCGAC40550.023.3042645
TCGACGA42550.023.2135687
TTCACGC253350.022.5307967
AACGCTA253000.022.02837423
TCACGCT257600.022.0018548
ACGCTAC257800.021.6007724
CGGTATA60600.021.4601220
CGCTACC261400.021.27746225
AAAACGC276050.020.25422321
CGACGAG47000.019.675288
AAACGCT291700.019.24469822
ACGCTCA301150.019.13390710
GAAAACG291400.018.8398420
CACGCTC306000.018.698299
CGTGGTT306750.018.33763741
TCGTGGT315300.017.73333440
ACCGCGT49050.017.7061966
GTTTTCG226850.016.91116545
ATGACGG74000.016.8443816
Produced by FastQC (version 0.11.5)