FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51343435
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCG153400.012.0056081
ATAGGGG146150.011.86803438
GGCATAG143750.011.29928335
GGAGGTC205600.011.2881064
GGGAGGT216900.011.1780993
GTTGTCG28150.011.02902226
AGCTTAG148750.010.97993945
TCAGACG93100.010.954532
CGACCCG170750.010.6103616
TGGCGAC176900.010.5114833
GGCGACC171700.010.5014134
GTGGCGA173300.010.4838352
GCGACCC173850.010.4744045
TCTATAG265900.010.1341573
GAGCTTA165550.09.98799744
AACTCTA114300.09.72301645
TGGCATA170850.09.71768834
TTCTATA290300.09.6398282
TAATCGG77000.09.6181324
CTATAGT279650.09.5789454
Produced by FastQC (version 0.11.5)