FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43780751
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG537250.12271374696153567No Hit
CTCGGTTTTATTCTTATTGCTCAGGCTTGAGTGCACTGGCCTCTTCTCAGC483620.11046407129927945No Hit
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATA459210.10488856164207873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG60450.030.33410532
CCCGGTT89150.030.015451
CGTCCGC64450.028.48637233
TCGTATG79150.023.45162245
CCGGTTC104700.023.2514722
GCCGGCT127300.023.178891
AGTCACG82350.022.54037928
CGCACAT82150.022.32132137
TTGCGTC70150.022.0984343
GTCCGTT38800.021.94371
GTCCGCA85250.021.82634434
CCGCACA85650.021.64560336
CCCCCCT27500.021.5413461
TAGCCCC97700.021.41698339
ACGCTCA129550.021.01480110
ACACGTC89900.020.97326913
TCCGCAC89050.020.97075535
AACGCTA129250.020.74990523
TCACGCT133800.020.5823618
ACGCTAC131050.020.48206924
Produced by FastQC (version 0.11.5)