FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43780751
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG63550.027.82252144
GGTCGCC62400.027.79452343
TGGTCGC67350.025.8185242
TCTCGGT77400.022.90214736
ATCTCGG78200.022.72541235
TCGCCGT74800.022.61542345
GTCGTGT78000.022.59171113
TCGTGTA78200.022.13054714
CTCGGTG80900.022.05028337
CGTCGTG79600.022.0246912
GCGTCGT81150.021.77040511
TCGGTGG85700.021.10399638
TGTAGGG83250.020.94921117
CGGTGGT86600.020.46905339
GTAGGGA83850.020.2082518
GATCTCG94800.018.72246734
GTGGTCG96700.018.098541
AGTGTAG98850.017.82028828
GTGTAGG100500.017.53284316
AGCGTCG101600.017.52131510
Produced by FastQC (version 0.11.5)