FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55430557
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG866460.15631450356885282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA212050.021.46562223
ACGCTAC214000.021.22796624
CGCTACC221400.020.50828625
TCACGCT237950.019.7149478
AAAACGC235800.019.43689221
GCCGGCT243750.018.5925541
GAAAACG250300.018.32025520
CCGGCTT281050.018.22922
AAACGCT252250.018.13362322
TTCACGC263700.018.0031137
ACGCTCA281500.017.27235810
AGACCGC101450.016.8109574
CACGCTC282950.016.7385439
ACCGCGT103950.016.4499426
CGGCTTC332900.016.0653823
CGTGGTT289300.015.87362341
CAGACCG109100.015.8178073
TTCGACG25100.015.7766286
GACCGCG109100.015.6321865
GTTTTCG297350.015.40605145
Produced by FastQC (version 0.11.5)