FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55430557
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1014230.18297308468323709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG273550.018.5971645
GGCATAG307450.016.49479535
ATAGGGG311150.016.32757638
GAGCTTA320500.015.85882344
GGGAGGT469650.015.4850133
GTTGTCG31800.015.14098626
GGAGGTC477900.014.9304784
TGGCATA343000.014.86391834
GGTCACT470550.014.7750457
GTCACTC479750.014.5387398
ACTCTCC495600.014.2013111
CATAGGG373650.013.69282237
AGGTCAC521550.013.6794856
GAGGTCA525800.013.6430755
GCATAGG391050.013.11232136
TGGGAGG414300.012.9643892
CACTCTC550400.012.75463310
CACCCGG149750.012.562021
TCACTCT567950.012.4869449
CTGGGAG500500.011.9860511
Produced by FastQC (version 0.11.5)