FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49895859
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG532010.10662407876373067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA193000.024.45552823
ACGCTAC196350.024.03883724
CGCTACC200350.023.5029925
AAAACGC210600.022.49645421
TCACGCT220650.022.0516438
AAACGCT227450.020.94918422
GAAAACG226600.020.93776320
ACGCTCA247900.020.28100210
TTCACGC252900.019.4263947
GTTTTCG259750.018.3462545
CACGCTC269450.018.274949
TTCGACG24050.018.239296
TCGTGGT266700.017.8400940
GTCCGTT39750.016.6537061
GTTAGTT129200.016.43682344
CGTGGTT294450.016.21991541
CTACCTC293950.016.17246427
TACCTCT300300.015.92048728
TCGGCGC91750.015.53258545
TTCGGCG93350.015.42318544
Produced by FastQC (version 0.11.5)