FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49895859
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG150250.015.17356945
GGCATAG164900.013.76561935
ATAGGGG177000.012.9773138
GAGCTTA178750.012.82845544
CATAGGG184950.012.38284437
TGGCATA194000.011.96749234
TAGCGTA10550.011.941637
GTCACTC258450.011.6423868
GCATAGG200900.011.41088836
GGGAGGT289100.011.3706833
ACTCTCC275800.011.056869511
GGTCACT273050.010.9371057
ATAGCGT12250.010.83530536
GGAGGTC293850.010.6589414
GAGGTCA292650.010.6341645
AGGTCAC287150.010.6266256
ACCCGGC86500.010.4317685
ATAGCTC80000.010.32087439
TCAGACG99200.010.1834232
CACCGAA181500.010.09347245
Produced by FastQC (version 0.11.5)