FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52279946
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG721030.13791712791746188No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT552190.1056217617363262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG25200.022.3163076
AGACCGC147550.016.983634
TCACGCT249000.016.8124078
ACGCTAC241600.016.73217224
AACGCTA241400.016.71780623
CGGTATA48150.016.44510820
GTTAGTT168250.016.20444544
TCGACGA35050.016.1097
CGCTACC252750.015.91387125
GAACGAT162350.015.76799945
TTCACGC274500.015.3571317
CAGACCG166800.015.3067983
ATGCGCG32050.015.23064644
ACCGCGT168150.015.0902046
GCCGGCT270300.015.0883311
AAAACGC272500.014.92521621
CCCGTAT31850.014.7584871
CGACGAG36750.014.7517178
CCGGCTT305500.014.7487162
GACCGCG171700.014.7126995
Produced by FastQC (version 0.11.5)