FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47080889
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA537940.11425867510700574No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG507190.10772736258229958No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG486550.10334341817547243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG188300.013.72027445
GGGAGGT270850.012.8184543
GGAGGTC271400.012.625744
GGCATAG204500.012.57718335
ATAGGGG205650.012.48379938
GTCACTC274450.012.3517688
GAGGTCA285800.012.2475065
GAGCTTA217950.011.93037944
GGTCACT284500.011.8604467
AGGTCAC295650.011.7098886
TAGCGTA7000.011.572103537
ACTCTCC298150.011.36012211
TGGCATA238450.010.90905934
TCACTCT325150.010.81189259
AGGGATC143000.010.5424832
TCAGACG97100.010.335222
ATATAGC37500.010.317856
GTTGTCG32300.010.31413326
CATAGGG253550.010.19680837
CACTCTC334900.010.18071510
Produced by FastQC (version 0.11.5)