FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47588562
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1067290.22427448007359416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC245500.022.1615074
ACCGCGT244300.021.9937366
GAACGAT248600.021.81178745
CAGACCG254450.021.5588593
GACCGCG250200.021.3854835
TTCGACG23750.021.2213276
CCGCGTT254050.021.1142277
CGCGTTC255350.021.0328378
TCAGACC302100.018.815312
AGAACGA296400.018.37786944
GCGTTCT305300.017.7092639
AATACGG308800.017.45768732
ATACGGA312900.017.2001733
CAATACG321400.016.98317731
TCGACGA30100.016.8939067
TACGGAG317650.016.84380334
CTCAGAC374650.016.6693951
AAGAACG329850.016.44596343
AACGCTA153400.016.3396423
ACGCTAC156250.016.14243924
Produced by FastQC (version 0.11.5)