FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51962514
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1319860.2540023371463513No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG557000.10719265815352969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC318100.021.0351684
CAGACCG323450.020.805493
GACCGCG340900.019.5754995
GAACGAT339600.019.43586245
ACCGCGT346550.019.3154496
CCGCGTT361500.018.4917357
CGCGTTC373650.017.9446338
TCAGACC387550.017.706832
GCGTTCT391350.017.2135249
AGAACGA389000.016.93842144
GTTAGTT148950.016.92111444
ACGCTAC227500.016.72362724
AACGCTA228550.016.66648523
TACGGAG392450.016.41365434
AATACGG398050.016.36926732
ATACGGA397600.016.36515833
CTCAGAC459000.016.2730831
CGCTACC234200.016.2355925
TCACGCT251500.015.6918778
CAATACG419800.015.58012331
Produced by FastQC (version 0.11.5)