FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51962514
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG908580.17485297189431598No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA541930.10429249054424118No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG528030.1016174852510023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG276600.016.39709545
GTCACTC409600.014.7417698
AGGTCAC418850.014.7333816
GGGAGGT440250.014.6078193
GGCATAG318900.014.20087635
GAGGTCA439000.014.1063255
ATAGGGG322900.014.04259838
GGAGGTC443650.013.9642754
GAGCTTA323750.013.95794344
ACTCTCC447200.013.51826211
TCAGACG112500.013.138152
TGGCATA352550.012.96321234
GGTCACT477050.012.7048597
TCACTCT486250.012.4826279
TGGGAGG397200.012.2549212
CACTCTC505550.011.90816210
CATAGGG389250.011.73868837
GCATAGG407150.011.23309536
CTCCCCA423500.011.05193414
CACCCGG138100.011.0280041
Produced by FastQC (version 0.11.5)