FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56158028
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1209530.21537971383183185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT276750.021.81482945
AGACCGC285550.021.1801154
CAGACCG301050.020.179513
GACCGCG300550.020.1153135
ACCGCGT302500.020.0586096
CCGCGTT314300.019.3055347
CGCGTTC317350.019.134178
AGAACGA330350.018.32981744
GCGTTCT352500.017.3027739
AATACGG349350.017.29307232
TCAGACC359650.017.291932
ATACGGA348150.017.10056733
TACGGAG347550.016.98118634
TTCGACG17250.016.5637976
CTCAGAC409750.016.503091
CAATACG373200.016.24902731
GTCCGTT46000.016.0998691
AAGAACG381600.015.87986243
ACGGAGA381800.015.546452535
CCCAATA405150.015.13373829
Produced by FastQC (version 0.11.5)