FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56158028
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG800140.14248007426471598No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN662780.11802052593442205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG239750.017.12889145
GTCACTC350800.015.0233478
GGCATAG278250.014.89725335
ATAGGGG282250.014.74178438
GAGCTTA281450.014.71092544
GGTCACT359550.014.6322067
AGGTCAC378750.014.1394616
GGGAGGT402800.014.0192763
ACTCTCC378550.013.95889411
CATAGGG306750.013.56484837
TGGCATA307900.013.55802534
GGAGGTC400350.013.48131754
GTTGTCG30050.013.32966126
GAGGTCA413250.013.2364695
GCATAGG323450.012.89217636
TCACTCT434800.012.2814769
TGGGAGG355750.012.2261792
CACTCTC444900.011.81606310
TAGGGGA467550.011.242928539
GCTCTGT481350.011.09030422
Produced by FastQC (version 0.11.5)