FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51045129
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG743740.14570244302840335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA222600.018.99277523
ACGCTAC223050.018.94479624
AGACCGC113550.018.8830134
TCACGCT233950.018.588298
ACGTTCG23800.018.3381986
CGCTACC232550.018.15173525
CAGACCG120150.017.9207173
AAAACGC237550.017.86255821
GTCCGTT44050.017.7490941
TACGGTA85100.017.55504244
GCCGGCT252500.017.2489661
GAAAACG245400.017.2361520
AAACGCT256950.016.56659922
GTTTTCG255500.016.53743245
CGTGGTT250600.016.42932741
TTACGGT92000.016.36083643
TTCACGC274250.016.0619377
ACGGTAC93800.016.02274945
GTTAGTT148200.015.57623744
TCGTGGT272000.015.34363340
Produced by FastQC (version 0.11.5)