FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47177287
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1406750.2981837425284756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT246600.027.55159445
AGACCGC295050.023.1548354
ACCGCGT295700.023.0809826
GACCGCG297850.022.9598275
AGAACGA302900.022.4469144
CCGCGTT304250.022.420197
CGCGTTC307900.022.1834268
CAGACCG308600.022.1527353
TCAGACC343250.020.1656072
AATACGG338950.020.10073932
ATACGGA343950.019.79534533
TACGGAG349400.019.49935234
GCGTTCT353300.019.4026379
AAGAACG357450.019.0218243
CAATACG366150.018.59446731
ACGGAGA376100.018.16276435
CTCAGAC409900.018.0806921
CCCAATA386100.017.76154129
CGGAGAG398500.017.07976536
CGTTCTC415100.016.74702610
Produced by FastQC (version 0.11.5)