FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47177287
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG148550.015.64503945
GGCATAG163500.014.14721135
ATAGGGG175600.013.27489938
GGTCACT247400.012.8683477
GTCACTC252050.012.8183168
CATAGGG184600.012.68863737
GAGCTTA185600.012.655272544
GCATAGG188650.012.39236536
GTTGTCG28000.012.37471326
TGGCATA193100.012.09454134
ACTCTCC273100.011.79746411
GGGAGGT313450.011.5060233
AGGTCAC299350.011.1161636
ACCCGGC89250.011.0917815
TGTCGTT31750.011.0549128
GGAGGTC300200.010.9946134
GAGGTCA309850.010.7101945
ATAGCTC87600.010.40267539
TCACTCT329250.010.0383759
AGCATAG88650.09.9484745
Produced by FastQC (version 0.11.5)