FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49733184
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT895290.18001863705328017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG185900.022.7730335
AGACCGC185900.022.7723244
ACCGCGT191750.022.054716
CAGACCG200350.021.3772433
CCGCGTT215000.019.6488597
TTCGACG25350.019.6107046
CGCGTTC221450.019.096848
CGGTATA39700.018.35785920
TCAGACC247850.017.5707952
GCGTTCT260350.016.3215079
CTCAGAC312050.015.7091141
AACGCTA140950.015.4047523
ACGCTAC142200.015.28614324
TCGACGA32950.015.22403057
CGCTACC146150.014.90606125
TTCGGCG90050.014.89410444
CTACACG41500.014.204945
TCGGCGC95200.014.18075445
GTCCGTT37800.014.1050681
CCGTACG96500.013.9159914
Produced by FastQC (version 0.11.5)