FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65141786
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG783980.12034978592696247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC181200.018.6956045
AGCTTAG244350.017.78198245
TCACCCG201750.017.3697173
GGCATAG251350.017.24163635
ACGGACA206700.016.55526518
CGGACAG210950.016.12666119
CGGACAC210100.016.09257113
GAGCTTA276200.015.82890744
CACCCGG219500.015.68886854
ATAGCTC207450.015.54103939
TGGCATA283200.015.41367834
ACACGGA221300.015.36113316
CATAGGG285050.015.27656437
CCGGACA224100.015.25765612
ACTCTCC393600.015.245211
GTCACTC406050.014.6225688
GGTCACT403300.014.5995957
GACACGG233500.014.56699715
TAGCGTA21450.014.47060137
CACGGAC237450.014.41102317
Produced by FastQC (version 0.11.5)