FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44845222
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC133900.018.1931215
TCACCCG138600.017.7862343
CGGACAC149750.016.13116613
CACCCGG156000.015.9328664
CCGGACA153350.015.89936412
CGGACAG151850.015.75997719
GTTGTCG24100.015.41287126
ACACGGA156200.015.40787516
AGCTTAG124200.015.21658845
ATAGCTC154050.014.97531339
ACGGACA165650.014.93569118
GACACGG163900.014.73885715
GGCATAG135200.013.84842535
GCCCGGA179100.013.73872910
TGATAGC173850.013.5804337
TAGCTCT171200.013.5406240
CACGGAC182950.013.3636917
TAGCGTA16050.013.33446237
GGACACG182450.013.20332414
TAGCATA101450.012.8468229
Produced by FastQC (version 0.11.5)