FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48549078
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG718460.14798633251078425No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA695190.14319324457613797No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG508750.10479086750112948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT345100.015.9147963
GGAGGTC343750.015.7625844
GAGGTCA373950.014.5501535
GTTGTCG43700.013.94896926
ATATAGC51050.013.9279176
AGCTTAG277900.013.53487545
GGTCACT400100.013.0922877
GTCACTC403300.013.0553968
AGGTCAC416950.013.0001296
ATAGGGG301100.012.73816338
ACTCTCC418050.012.52959511
GGCATAG305750.012.48467835
TGGGAGG326450.012.2940042
GAGCTTA311200.012.10189544
CGTTTCG6100.011.80205544
CACCGAA323100.011.79468345
TCACTCT451350.011.6454669
TGGCATA333400.011.55445734
TCAGACG93750.011.5486852
TATAGCT64400.011.4950567
Produced by FastQC (version 0.11.5)