FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40603930
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG13300.016.9149136
GTCCGTT30300.016.87371
TCGGCGC78600.016.22905245
TTCGGCG80900.016.04580544
AACGCTA125200.014.88181823
ACGCTAC125300.014.87049124
CTACACG56600.014.78628745
CTCCGTT66900.014.7460291
GTTAGTT130150.014.71023644
CCGTACG88000.014.5462484
CGCTACC128850.014.44430225
TACGGTA71100.014.27056544
GCCGGCT135350.014.2609891
CGTACGC89950.014.2309055
ACGCTCA141800.014.1359510
GCACTAC44400.014.09085225
TCACGCT143850.013.950078
ATGCGCG21400.013.87696244
CGCCCCG93500.013.71807723
CCTTACG74800.013.62458841
Produced by FastQC (version 0.11.5)