FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48595922
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC82600.013.7906445
GGCATAG134450.013.49734635
TCAGACG85500.013.2208842
AGCTTAG144500.012.92588445
GTTGTCG29500.012.64495926
ATAGGGG146450.012.55850438
CACCCGG93600.012.5258844
CCGTACG22200.012.5028024
TCACCCG94250.012.0401993
GTCACTC194650.012.0132628
CGTACGC23700.011.9924755
CGGACAC93150.011.82443513
GGTCACT200050.011.7766267
GGAGGTC207850.011.74080854
TAGCGTA11000.011.71205737
CATAGGG155400.011.64942437
ACGGACA97150.011.588078518
GGGAGGT230600.011.5177073
GCATAGG159400.011.45462636
CAGACGT105850.011.2871833
Produced by FastQC (version 0.11.5)