FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47506469
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA71300.021.90017523
ACGCTAC74400.020.89711824
ACCGCGT90750.020.010026
CGCTACC78500.019.7197725
GACCGCG94050.019.2362585
AGACCGC95600.018.9243724
GAACGAT96550.018.82738945
CCGCGTT96550.018.781287
CGCGTTC100550.018.0338738
AAAACGC90750.017.6519721
AATACGG110800.016.57109832
TTCGGCG76250.016.11080244
CAGACCG114650.016.0154613
ATACGGG52950.015.850430533
GAAAACG103600.015.65735420
AGAACGA118500.015.60694244
TACGGGG55550.015.5539734
GTCCGTT39550.015.2076151
TCGGCGC80100.015.13865345
AAACGCT106200.014.89333522
Produced by FastQC (version 0.11.5)