FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47506469
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG112500.014.67885345
GGCATAG117900.014.0270435
CATAGGG126650.013.02246237
GCATAGG134600.012.40370336
ATAGGGG136100.012.36624238
ACCCGGC88200.012.1167455
GAGCTTA139850.012.09773244
ACGGACA89000.011.57864918
ATAGCTC87200.011.30187639
TGGCATA148450.011.27634334
GGGAGGT244100.010.2955713
ACTCTCC219500.010.20919911
GTCACTC221200.010.1917038
TAGCTCT98150.010.13269440
CGGACAC101000.010.06876513
TGATAGC104100.09.96424837
CCGGACA106700.09.86833212
TCAGACG82700.09.7940122
CGGACAG103300.09.77976519
GGTCACT225200.09.7410117
Produced by FastQC (version 0.11.5)