FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43892085
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG799750.18220825007515593No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT620960.14147425441284003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA173250.024.07903523
ACGCTAC175300.023.77212724
CGCTACC184050.022.59306525
TCACGCT195200.021.9434328
AAAACGC192500.021.76327721
ACGCTCA210500.021.08524110
TTCGACG22800.021.0163966
GAAAACG203950.020.59636120
GCCGGCT219150.020.5179861
TGCGCGT45700.020.11311345
AAACGCT213800.019.57444622
CACGCTC224100.019.3122639
TTCACGC240350.018.1114947
TCGACGA27950.017.788037
ACGGTAC111600.017.64341445
ACGTTCG27300.017.634566
ATGCGCG52450.017.63263344
TCGTGGT244900.017.08875540
CGTGGTT245950.017.06975641
CGGCTTC290850.016.947773
Produced by FastQC (version 0.11.5)