FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42131170
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG33700.023.7009836
TCGACGA37600.021.6616617
CGACGAG37850.020.7458178
GTTCGAC42300.018.6695845
CGGTATA55400.018.3568720
CCCGTAT26450.016.2679021
GTCCGTT33300.015.6275771
CCCCCCT32450.015.4815361
CTCCGTT58850.014.9676681
GCGGACT47350.014.8727842
GTCCGGT31600.014.4008511
GCACTAC56450.014.26885325
ATGACGG68450.014.2326416
CTCGGTT68650.014.0451741
GTCCGAT22300.014.0421561
CCCGGAT78950.013.8678141
GACGGTA73400.013.7632118
GTCCCGT36000.013.7045731
GTTCCTA76900.013.40026115
GTTCGCT36850.013.3273211
Produced by FastQC (version 0.11.5)